门静脉血氨对猪肝尿素循环和糖异生的影响

doi:10.11843/j.issn.0366-6964.2017.01.011

Abstract

Abstract:

This experiment was conducted to study the effect of portal ammonia on urea cycle and gluconeogenesis in liver of pig. Eight pigs (Duroc×Landrace×Yorkshire), with the average weight of 20 kg, were randomly assigned to two treatments with 4 pigs in each treatment. Pigs were surgically implanted with catheter in the portal vein and hepatic vein. Then 65 and 35 mmol•L^-1 of ammonium chloride (NH₄Cl) were infused into liver by portal catheter. Serum samples were collected to analyze metabolites based on gas chromatography-mass spectrometry metabolomics. Liver samples were collected to examine gene expression and enzyme activity in urea cycle and gluconeogenesis. The results showed that, compared with low concentration NH₄Cl, the infusion of high concentration NH₄Cl significantly increased the content of urea, glucose and glucose-6-phosphate(P<0.05), the content of alanine, glutamate and aspartate were significantly decreased (P<0.05); addtionally, high concentration NH₄Cl significantly upregulated the expression of carbamoyl-phosphate synthase 1 (CPS1), ornithine carbamoyl-transferase (OTC), arginase 1 (Arg1), phosphoenolpyruvate carboxykinase (PCK) and glucose-6-phosphatase (G6PC), meanwhile the corresponding enzyme activities increased 50.77%, 44.47%, 41.24%, 46.99% and 54.57%（P<0.05）, respectively. These results indicated that, with the ammonia load increased into liver, the urea cycle was increased and the additional nitrogen was supplied by amino acid metabolism (alanine, aspartate, glutamate), of which the carbon skeleton were used for hepatic gluconeogenesis.

CLC Number:

S828
S852.2

BAO Zheng-xi, LI Lu-lu, WANG Tong-xin, LIU Shuang, ZHANG Ping, HUANG Fei-ruo. Effect of Portal Ammonia on Urea Cycle and Gluconeogenesis in Liver of Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 91-98.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.01.011

https://www.xmsyxb.com/EN/Y2017/V48/I1/91

References

［1］BLACHIER F, MARIOTTI F, HUNEAU J F, et al. Effects of amino acid-derived luminal metabolites on the colonic epithelium and physiopathological consequences ［J］. Amino Acids, 2007, 33(4): 547-562.
［2］WINDMUELLER H G. Glutamine utilization by the small intestine ［J］. Adv Enzymol Relat Areas Mol Biol, 1982, 53:201-237.
［3］FOUILLET H, JUILLET B, BOS C, et al. Urea-nitrogen production and salvage are modulated by protein intake in fed humans: results of an oral stable-isotope-tracer protocol and compartmental modeling ［J］. Am J Clin Nutr, 2008, 87(6): 1702-1714.
［4］STOLL B, HENRY J, REEDS P J, et al. Catabolism dominates the first-pass intestinal metabolism of dietary essential amino acids in milk protein-fed piglets ［J］. J Nutr, 1998, 128(3): 606-614.
［5］HÄUSSINGER D. Ammonia, urea production and pH regulation［M］. 3rd Edition. The textbook of hepatology: from basic science to clinical practice. Eds Rodes J, Benhamou JP, Blei A, Reichen J and Rizzetto M. Wiley-Blackwell, 2007: 181-192.
［6］黄飞若. 猪营养代谢与表观遗传学［M］. 北京：化学工业出版社, 2016.
HUANG F R. Porcine nutrition metabolism and epigenetic ［M］. Beijing: Chemical Industry Press, 2016.（in Chinese）
［7］MILANO G D, HOTSTON-MOORE A, LOBLEY G E. Influence of hepatic ammonia removal on ureagenesis, amino acid utilization and energy metabolism in the ovine liver ［J］. Br J Nutr, 2000, 83(3): 307-315.
［8］YANG D, HAZEY J W, DAVID F, et al. Integrative physiology of splanchnic glutamine and ammonium metabolism ［J］. Am J Physiol Endocrinol Metab, 2000, 278(3): E469-E476.
［9］BROSNAN J T, BROSNAN M E, YUDKOFF M, et al. Alanine metabolism in the perfused rat liver. Studies with (15)N ［J］. J Biol Chem, 2001, 276(34): 31876-31882.
［10］LI L, ZHANG P, BAO Z, et al. PGC-1α promotes ureagenesis in mouse periportal hepatocytes through SIRT3 and SIRT5 in response to glucagon［J］. Sci Rep, 2016, 6: 24156.
［11］YIN Y L, HUANG R L, LI T J, et al. Amino acid metabolism in the portal-drained viscera of young pigs: effects of dietary supplementation with chitosan and pea hull［J］. Amino Acids， 2010, 39(5): 1581-1587.
［12］郑培培，包正喜，李鲁鲁，等．肝脏营养物质代谢的仔猪肝-门静脉血插管技术的建立［J］．动物营养学报，2014，26(6)：1624-1631．
ZHENG P P, BAO Z X, LI L L, et al. Establishment of techniques for implanting hepatic-portal catheters in piglets for studying hepato-nutrients metabolism ［J］. Chinese Journal of Animal Nutrition, 2014, 26(6): 1624-1631. (in Chinese)
［13］BUSH J A, WU G, SURYAWAN A, et al. Somatotropin-induced amino acid conservation in pigs involves differential regulation of liver and gut urea cycle enzyme activity［J］. J Nutr, 2002, 132(1): 59-67.
［14］贾逸敏. 母猪低蛋白日粮对新生仔猪肝脏糖异生和肝脏线粒体功能的影响及其表遗传机制［D］. 南京: 南京农业大学, 2012.
JIA Y M. Effect of maternal low protein dietary on hepatic gluconeogenesis and mitochondrial function in newborn piglets and its epigenetic mechanisms ［D］. Nanjing: Nanjing Agricultural University, 2012. (in Chinese)
［15］LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method ［J］. Methods, 2001, 25(4): 402-408.
［16］VOLLMAR B, MENGER M D. The hepatic microcirculation: mechanistic contributions and therapeutic targets in liver injury and repair ［J］. Physiol Rev, 2009, 89(4): 1269-1339.
［17］BROSNAN J T, BROSNAN M E. Hepatic glutaminase-a special role in urea synthesis?［J］. Nutrition, 2002, 18(6): 455-457.
［18］HÄUSSINGER D. Regulation of hepatic ammonia metabolism: the intercellular glutamine cycle ［J］. Adv Enzyme Regul, 1986, 25: 159-180.
［19］HÄUSSINGER D. Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfused rat liver ［J］. Eur J Biochem, 1983, 133(2): 269-275.
［20］MORRIS S M Jr. Regulation of enzymes of urea and arginine synthesis ［J］. Annu Rev Nutr, 1992, 12: 81-101.
［21］LOBLEY G E, CONNELL A, LOMAX M A, et al. Hepatic detoxification of ammonia in the ovine liver: possible consequences for amino acid catabolism ［J］. Br J Nutr, 1995, 73(5): 667-685.
［22］NISSIM I, YUDKOFF M, BROSNAN J T. Regulation of ［15N］ urea synthesis from ［5-15N］ glutamine. Role of pH, hormones, and pyruvate ［J］. J Biol Chem, 1996, 271(49): 31234-31242.

[1]	NIU Naiqi, ZHAO Runze, ZONG Wencheng, LIU Xiance, LIU Hai, SHI Guohua, JING Xitao, ZHANG Longchao. Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 79-86.
[2]	ZHU Xueli, ZHANG Longchao, WANG Lixian, PU Lei, LIU Xin. Association Analysis of AQP9 and RPS10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 87-98.
[3]	SHI Shengjie, WANG Liguang, GAO Lei, CAI Chuanjiang, HE Weixian, CHU Guiyan. Effect of miR-24-3p on Estradiol Synthesis in Porcine Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 169-178.
[4]	YANG Kai, LU Zhuoda, HE Jian, ZHANG Ruiqi, WANG Suqing, LI Kebiao, ZHAO Yunxiang, ZHU Xiaoping, GUO Jinbiao. The Population Effect of Commercial Pigs on the Accuracy of Genomic Selection for Carcass Traits in Duroc Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4943-4951.
[5]	LI Chao, ZHAO Xueyan, WANG Yongjun, WANG Yanping, REN Yifan, LI Jingxuan, WANG Huaizhong, WANG Jiying, SONG Qinye. Analysis on the Structural Composition and Function of Bacterial Microbiota of Caecum and Colon in Laiwu and DLY pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5033-5045.
[6]	XU Tingting, QI Fenfang, HUANG Shihui, NIU Xi, LI Sheng, RAN Xueqin, WANG Jiafu, XIE Jian. Study of the Polymorphisms of Structural Variation and the Expression of MAP3K4 gene in Xiang Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5046-5055.
[7]	XUE Hongyan, YANG Mengyu, YANG Huan, DONG Lijun, CAI Xiaqing, ZHAO Zemin, WANG Xianzhong. The Role of ALOX15B-JNK in Heat Stress-induced Oxidative Stress and Apoptosis of Sertoli Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5056-5065.
[8]	HU Ziping, WANG Ligang, ZONG Wencheng, HOU Renda, SU Yanfang, NIU Naiqi, WANG Lixian, WANG Yuan, ZHNAG Longchao. Genetic Structure Analysis of Jianbai Xiang Pig Population Based on Genomic SNP and ROH [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4117-4125.
[9]	JI Zhengyu, NI Mengru, ZHANG Zhaobo, ZHAO Gan, HUANG Zan, LI Pinghua, HUANG Ruihua, HOU Liming. Research on Adipogenic Capacity and Gene Expression Pattern of in Vitro FAPs Cells Derived from Longissimus Dorsi Muscle of Suhuai Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4126-4142.
[10]	BI Huan, QIN Hai, YUAN Wei, ZHANG Yudan, ZHANG Yiyu, CHEN Wei. Effect of Knocking down TYRP1 Gene on Melanin Production in the Xiang Pig Epidermal Melanocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4143-4153.
[11]	LIU Pan, LI Ruiqi, TAN Zhankun, WANG Yifei, CHEN Xiaochen, HE Weixian, DU Renrang, MA Jian, CHU Guiyan, CAI Chuanjiang. Effects of High Fiber Diet on Growth Performance, Meat Quality and Intestinal Microbiome of Growing-finishing Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4247-4259.
[12]	LI Pinghui, PU Guang, WANG Zhongyu, ZHOU Wuduo, NIU Peipei, WU Chengwu, HOU Liming, HUANG Ruihua, LI Pinghua. Effect of Dietary Fiber Level on Blood and Intestinal Immune Indexes of Meishan Pigs and Preliminary Analysis of Its Mechanism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4260-4277.
[13]	WANG Hui, FENG Baoliang, WU Dan, XIANG Guangming, WANG Nan, MU Yulian, LI Kui, LIU Zhiguo. Research Progress of CD163 Gene and Disease-Resistant Breeding on Porcine Reproductive and Respiratory Syndrome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3127-3138.
[14]	ZHANG Wanfeng, ZHAO Tianzhi, LI Jiao, YOU Ziwei, YANG Yang, CAI Chunbo, GAO Pengfei, CAO Guoqing, GUO Xiaohong, LI Bugao. Study on NR2F2 Gene Regulating Proliferation and Apoptosis of Porcine PK15 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3242-3251.
[15]	LIAN Yuju, ZHANG Zhiyuan, LIAO Xiaobo, WEI Hongjiang, YIN Yulong, LIU Mei. Breeding Methods and Application Progress of Medical Miniature Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2667-2682.

Effect of Portal Ammonia on Urea Cycle and Gluconeogenesis in Liver of Pig

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments